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p akt  (Cell Signaling Technology Inc)


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Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc p akt
    P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+akt/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pmc13018931-113-24-27
    Average 99 stars, based on 17147 article reviews
    p akt - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Immunophilin binding agents and uses thereof
    Article Snippet: .. MW Antibody 1 Source Species Dilution Antibody 2 Source Species Dilution 100-55 P-AKT[S473] CST- Rabbit 1:1000 AKT CST- Mouse 1:1000 4060 2020 55-25 P-S6[S240/244] CST- Rabbit 1:2000 S6 CST- Mouse 1:1000 5364 2317 100+ P-ULK1[S757] Rabbit 1:1000 25− P-4EBP1[T37/46] CST- Rabbit 1:1000 FKBP12 Ab58072 Mouse 1:500 2855 55-25 GAPDH 60004- Mouse 1:50000 S6 CST- Rabbit 1:1000 1-Ig 2217 100+ ULK1 Rabbit 1:1000 25− 4EBP1 CST- Rabbit 1:1000 9644 Primary antibodies were added as solutions in 5% BSA-TBST, and the membranes were incubated at 4° C. overnight (16 h). ..

    Article Title: A multifunctional injectable MPDA@MTIC–Co hydrogel platform for synergistic chemotherapy–photothermal therapy of postoperative glioblastoma
    Article Snippet: .. Membranes were incubated with primary antibodies against PI3K, AKT, and p-AKT (Cell Signaling Technology) overnight, followed by HRP-conjugated secondary antibodies. .. Signals were detected using ECL reagent (Thermo Fisher), with GAPDH as loading control.

    Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis
    Article Snippet: Twenty micrograms of protein from each sample were separated by 4% or 15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (4561083, Bio-Rad) and transferred to polyvinylidene difluoride membranes (1704156, Bio-Rad). .. After blocking with 5% BSA, the membranes were incubated overnight at 4°C with primary antibodies against: DSPP (1:2000, bs-10316R, Bioss), DMP1 (1:1000, 12359R, Bioss), p-AKT (1:2000, 4060S, CST), AKT (1:500, 60203-2-Ig, Proteintech), eNOS (1:500, ab76198, Abcam), and GAPDH (1:5000, AC026, Proteintech). ..

    Article Title: Single-stranded DNA in the bone microenvironment promotes prostate cancer bone metastasis via the ITGA6-FAK pathway.
    Article Snippet: .. Blots were incubated with primary antibodies described as the following: FAK (Forevertech Biotechnologies Co., Ltd, 20431, 1:1000), p-FAK (Tyr397) (CST, 3283S, 1:1000), AKT (CST, 9272S, 1:1000), p-AKT (CST, 4060S, 1:1000), ERK (CTS, 9102S, 1:1000), p-ERK (CST, 4370S, 1:1000), ITGA6 (proteintech, 27189-1-AP, 1:1000), ITGB4 (proteintech, 21738-1-AP, 1:1000), and GAPDH (Origene, TA802519, 1:5000). .. Secondary antibodies including Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Invitrogen, AB_228307) and Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Invitrogen, AB-228341) were subsequently applied.

    other:

    Article Title: Veronicastrum sibiricum (L.) Pennell extract alleviates inflammation-induced muscle atrophy through NLRP3 inflammasome regulation and mitochondrial function restoration
    Article Snippet: p-Akt , Rabbit monoclonal , 9271 , Cell Signaling Technology, Inc. , 1:1,000.

    Blocking Assay:

    Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis
    Article Snippet: Twenty micrograms of protein from each sample were separated by 4% or 15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (4561083, Bio-Rad) and transferred to polyvinylidene difluoride membranes (1704156, Bio-Rad). .. After blocking with 5% BSA, the membranes were incubated overnight at 4°C with primary antibodies against: DSPP (1:2000, bs-10316R, Bioss), DMP1 (1:1000, 12359R, Bioss), p-AKT (1:2000, 4060S, CST), AKT (1:500, 60203-2-Ig, Proteintech), eNOS (1:500, ab76198, Abcam), and GAPDH (1:5000, AC026, Proteintech). ..



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